Analytical Chemistry

High Performance Liquid Chromatography (HPLC)

High Performance Liquid Chromatography (HPLC) is a powerful analytical technique used for separating, identifying, and quantifying components in a mixture. In HPLC, the quality of the mobile phase, which includes water, is crucial for achieving accurate and reproducible results. Ensuring high-purity water is essential to minimize interference and maintain the integrity of chromatographic analyses.

What Is The Impact Of Water On HPLC?

The quality of water directly impacts the performance of HPLC. Impurities in water can lead to baseline noise, decreased
column efficiency, and inaccurate peak integration, affecting the reliability of analytical results and the overall chromatographic resolution.

What Types Of Contaminants In Water Can Affect HPLC Results?

Water impurities that can impact HPLC results include:

Ionic Contaminants

Impact:

Elevated levels of ions can interfere with HPLC separations.

Effect:

Excess ions may affect the baseline and peak shapes, leading to variations in chromatographic results. In reverse-phase HPLC, for example, interference from ions can impact the resolution and accuracy of compound separations.

Organic Contaminants

Impact:

The presence of organic impurities can affect the sensitivity and selectivity of HPLC analyses.

Effect:

In quantitative analyses, unintended organic compounds may introduce unwanted signals, affecting the accuracy of concentration measurements and potentially compromising the reliability of results.

Particulates

Impact:

Presence of particulate matter in HPLC solvents can cause column blockages.

Effect:

Particulates may lead to fluctuations in flow rates and affect the reproducibility of chromatographic peaks. This interference can compromise the precision of HPLC analyses.

Water Measurement Requirements
for HPLC

The requisite level of water purity is contingent upon the sensitivity of the applications in question. For routine analyses, Type Il water proves adequate, yet for highly sensitive work, maintaining extremely low levels of impurities becomes imperative. Achieving Total Organic Carbon (TOC) values below 2 ppb is particularly desirable for the most sensitivity demanding applications.

Water Quality Standards According to Industry Guidelines:

Method

Sensitivity

Resistivity
(MΩ.cm)

TOC
(ppb)

Filter (μm)

Bacteria
(CFU/mL)

Endotoxin

Nuclease

HPLC

General

<20

<0.2

<10

HPLC

High

>18

<0.2

<0.03

General

<50

<0.2

<10

High

18.2

<10

<0.2

<0.03

LC-MS

High

>18

<0.2

<0.03

Method

HPLC

Sensitivity

General

Resistivity (MΩ.cm)

TOC (ppb)

<20

Filter (μm)

<0.2

Bacteria (CFU/mL)

<10

Endotoxin

Nuclease

Method

HPLC

Sensitivity

High

Resistivity (MΩ.cm)

>18

TOC (ppb)

Filter (μm)

<0.2

Bacteria (CFU/mL)

Endotoxin

<0.03

Nuclease

Method

Sensitivity

General

Resistivity (MΩ.cm)

TOC (ppb)

<50

Filter (μm)

<0.2

Bacteria (CFU/mL)

<10

Endotoxin

Nuclease

Method

Sensitivity

High

Resistivity (MΩ.cm)

18.2

TOC (ppb)

<10

Filter (μm)

<0.2

Bacteria (CFU/mL)

Endotoxin

<0.03

Nuclease

Method

LC-MS

Sensitivity

High

Resistivity (MΩ.cm)

>18

TOC (ppb)

Filter (μm)

<0.2

Bacteria (CFU/mL)

Endotoxin

<0.03

Nuclease

Adhering to these water quality standards is crucial for achieving accurate and reproducible results in trace organic analyses. Type I water is recommended for applications requiring the highest level of sensitivity, while Type Il water may be suitable for routine analyses.

NOTES:

If removal of finer particulates (< 0.05 micron) is required, consider upgrading to CLS-5400 series systems.
Applications requiring Endotoxin filtration should purchase a CLF-5000-02E Endotoxin removal final filter.

CLS-3200

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Download Spec Sheet

CLïR 3200 High Purity Lab Water System with UV Oxidation

25.25

⅜" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 10 CFU / ml

< 1ppb

< 5 ppb

Countertop, Wall Mount

Electrochemistry, Gas Chromatography Mass Spectrometry (GC/MS), High Performance Liquid Chromatography (HPLC), Inductively Coupled Plasma Mass Spectrometry (ICP-MS), Trace Organics

Analytical Chemistry

CLS-5200-R-1-TOC

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CLïR 5200 Ultrapure Lab Water System with TOC Monitoring & UV Oxidation (Remote 120V)

14.4

21.6

14.7

⅜" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

< 5 ppb

2.5

Wall Mount, Remote Dispense

Electrochemistry, Gas Chromatography Mass Spectrometry (GC/MS), High Performance Liquid Chromatography (HPLC), Inductively Coupled Plasma Mass Spectrometry (ICP-MS), Trace Organics

Analytical Chemistry

CLS-5200-R-2-TOC

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CLïR 5200 Ultrapure Lab Water System with TOC & UV Oxidation (Remote 220V)

14.4

21.6

14.7

⅜" O.D. Tubing

220V/50Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

< 5 ppb

2.5

Wall Mount, Remote Dispense

Electrochemistry, Gas Chromatography Mass Spectrometry (GC/MS), High Performance Liquid Chromatography (HPLC), Inductively Coupled Plasma Mass Spectrometry (ICP-MS), Trace Organics

Analytical Chemistry

CLS-5200-S-1-TOC

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Download Spec Sheet

CLïR 5200 Ultrapure Lab Water System with TOC Monitor & UV Oxidation (Standard 120V)

14.4

21.6

14.7

½" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

< 5 ppb

2.5

Countertop

Electrochemistry, Gas Chromatography Mass Spectrometry (GC/MS), High Performance Liquid Chromatography (HPLC), Inductively Coupled Plasma Mass Spectrometry (ICP-MS), Trace Organics

Analytical Chemistry

System

Label

System Depth (in)

System Height (in)

System Width (in)

Dry Weight (lbs)

Inlet Connection

System Voltage

TOC Monitoring

0.05μ Ultrafiltration

Ultraviolet Oxidation

Effluent Purity

Effluent Microorganisms

Effluent Sodium

Effluent Chlorides

Effluent TOC

System Maximum
Output (lpm)

Volumetric Presets

Mounting

Laboratory Applications

CAtegory

CLS-3200

Get a quote

Download Spec Sheet

CLïR 3200 High Purity Lab Water System with UV Oxidation

25.25

⅜" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 10 CFU / ml

< 1ppb

< 5 ppb

Countertop, Wall Mount

Electrochemistry, Gas Chromatography Mass Spectrometry (GC/MS), High Performance Liquid Chromatography (HPLC), Inductively Coupled Plasma Mass Spectrometry (ICP-MS), Trace Organics

Analytical Chemistry

CLS-5200-R-1-TOC

Get a quote

Download Spec Sheet

CLïR 5200 Ultrapure Lab Water System with TOC Monitoring & UV Oxidation (Remote 120V)

14.4

21.6

14.7

⅜" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

< 5 ppb

2.5

Wall Mount, Remote Dispense

Electrochemistry, Gas Chromatography Mass Spectrometry (GC/MS), High Performance Liquid Chromatography (HPLC), Inductively Coupled Plasma Mass Spectrometry (ICP-MS), Trace Organics

Analytical Chemistry

CLS-5200-R-2-TOC

Get a quote

Download Spec Sheet

CLïR 5200 Ultrapure Lab Water System with TOC & UV Oxidation (Remote 220V)

14.4

21.6

14.7

⅜" O.D. Tubing

220V/50Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

< 5 ppb

2.5

Wall Mount, Remote Dispense

Electrochemistry, Gas Chromatography Mass Spectrometry (GC/MS), High Performance Liquid Chromatography (HPLC), Inductively Coupled Plasma Mass Spectrometry (ICP-MS), Trace Organics

Analytical Chemistry

CLS-5200-S-1-TOC

Get a quote

Download Spec Sheet

CLïR 5200 Ultrapure Lab Water System with TOC Monitor & UV Oxidation (Standard 120V)

14.4

21.6

14.7

½" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

< 5 ppb

2.5

Countertop

Electrochemistry, Gas Chromatography Mass Spectrometry (GC/MS), High Performance Liquid Chromatography (HPLC), Inductively Coupled Plasma Mass Spectrometry (ICP-MS), Trace Organics

Analytical Chemistry

The difference is CLïR.

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Applications

Analytical Chemistry

High Performance Liquid Chromatography (HPLC)

What Is The Impact Of Water On HPLC?

Ionic Contaminants

Organic Contaminants

Particulates

Water Measurement Requirementsfor HPLC

Method

Sensitivity

Resistivity (MΩ.cm)

TOC (ppb)

Filter (μm)

Bacteria (CFU/mL)

Endotoxin

Nuclease

Method

Sensitivity

Resistivity (MΩ.cm)

TOC (ppb)

Filter (μm)

Bacteria (CFU/mL)

Endotoxin

Nuclease

Method

Sensitivity

Resistivity (MΩ.cm)

TOC (ppb)

Filter (μm)

Bacteria (CFU/mL)

Endotoxin

Nuclease

Method

Sensitivity

Resistivity (MΩ.cm)

TOC (ppb)

Filter (μm)

Bacteria (CFU/mL)

Endotoxin

Nuclease

Method

Sensitivity

Resistivity (MΩ.cm)

TOC (ppb)

Filter (μm)

Bacteria (CFU/mL)

Endotoxin

Nuclease

System

Label

System Depth (in)

System Height (in)

System Width (in)

Dry Weight (lbs)

Inlet Connection

System Voltage

TOC Monitoring

0.05μ Ultrafiltration

Ultraviolet Oxydation

Effluent Purity

Effluent Microorganisms

Effluent Sodium

Effluent Chloride

Effluent TOC

System MaximumOutput (lpm)

Volumetric Presets

Mounting

Laboratory Applications

CAtegory

System

Label

System Depth (in)

System Height (in)

System Width (in)

Dry Weight (lbs)

Inlet Connection

System Voltage

TOC Monitoring

0.05μ Ultrafiltration

Ultraviolet Oxydation

Water Measurement Requirements
for HPLC

System Maximum
Output (lpm)

System Maximum
Output (lpm)

System Maximum
Output (lpm)

System Maximum
Output (lpm)

System Maximum
Output (lpm)