Biologic Chemistry

Cell Culture

Cell culture is a fundamental technique in life sciences that involves the growth and maintenance of cells outside their natural environment. The quality of water used in cell culture is critical for maintaining cell health, preventing contamination, and ensuring the success of experiments. High-purity water is essential to provide a suitable environment for cell growth and to minimize the risk of introducing contaminants.

What Is The Impact Of Water On Cell Culture?

Water quality significantly impacts cell culture outcomes. Contaminated water can lead to compromised cell viability, altered cell behavior, and contamination of cell cultures. High-purity water is essential for successful cell culture experiments, as cells are sensitive to various contaminants, including microorganisms and impurities.

What Types Of Contaminants In Water Can Affect Cell Culture?

Water impurities that can impact cell culture include:

Bacteria

Impact:

Bacterial contamination can adversely affect cell cultures.

Effect:

Bacteria thrive in cell culturing conditions, outgrowing cells of interest, causing changes in nutrient levels, media pH, and leading to contamination of previously pure cultures. This can compromise the success of cell culture techniques.

Endotoxins

Impact:

Endotoxins released by bacteria can affect various cell types.

Effect:

In cell culture, endotoxins stimulate immune responses, leading to changes in cell growth, function, and the production of recombinant proteins. Efficiency in cloning and other cellular processes can be reduced due to the presence of endotoxins.

Organic Compounds

Impact:

Small organic compounds in water can affect cell development.

Effect:

Organic impurities, such as humic acids and pesticides, provide uncontrolled nutrients for bacterial growth. Removal of these organic compounds from water used for cell culture preparation is crucial to avoid adverse effects on cell development.

Ionic Contaminants

Impact:

Ionic contaminants, particularly multivalent ions, must be kept low.

Effect:

Heavy metals like mercury and lead, which are cytotoxic, can negatively impact various cell types. Maintaining low levels of ionic contaminants is essential to ensure the success of cell culture techniques.

Water Measurement Requirements for Cell Culture

In cell culture applications, the quality of water is a critical factor that directly impacts experimental outcomes. Contaminated cultures and cell death can result from impurities in water, affecting downstream experiments. For cell culture, maintaining the highest purity standards with apyrogenic, Type I ultrapure water is crucial to ensure successful outcomes.

Water Quality Standards According to Industry Guidelines:

Selecting the appropriate water type is critical for your specific application. Mammalian cell culture, for instance, necessitates the use of apyrogenic, Type I ultrapure water. In contrast, Type II+ water is suitable for bacterial cell cultures, which exhibit lower sensitivity. Specifically, Type I water is recommended for the preparation of media and buffers to guarantee the absence of bacterial, yeast, and viral contaminants, ensuring optimal conditions for cell cultures.

Application

Bacterial Cell Culture

Resistivity (MΩ.cm)*

TOC (ppb)

<50

Filter (μm)

<0.2

Bacteria (CFU/ml)

Endotoxins (EU/ml)

Nucleases

Water Grade

Type II+

Application

Mammalian Cell Culture

Resistivity (MΩ.cm)*

>18

TOC (ppb)

<10

Filter (μm)

<0.2

Bacteria (CFU/ml)

Endotoxins (EU/ml)

<0.002

Nucleases

Not Detected

Water Grade

Type I

Application

Plant Tissue Culture

Resistivity (MΩ.cm)*

>18

TOC (ppb)

<10

Filter (μm)

<0.2

Bacteria (CFU/ml)

Endotoxins (EU/ml)

<0.002

Nucleases

Not Detected

Water Grade

Type I

Application

Histology

Resistivity (MΩ.cm)*

TOC (ppb)

<50

Filter (μm)

<0.2

Bacteria (CFU/ml)

Endotoxins (EU/ml)

Nucleases

Water Grade

Type II+

Application

Hydroponics

Resistivity (MΩ.cm)*

TOC (ppb)

<50

Filter (μm)

<0.2

Bacteria (CFU/ml)

Endotoxins (EU/ml)

Application
Resistivity (MΩ.cm)*
TOC (ppb)
Filter (μm)
Bacteria (CFU/ml)
Endotoxins (EU/ml)
Nucleases
Water Frade
Bacterial
Cell Culture
>1
<50
<0.2
<1
NA
NA
Type II+
Mamallian
Cell Culture
>18
<10
<0.2
<1
<0.002
Not Detected
Type I
Plant Tissue
Culture
>18
<10
<0.2
<1
<0.002
Not Detected
Type I
Histology
>1
<50
<0.2
<1
NA
NA
Type II+
Hydroponics
>1
<50
<0.2
<1
NA
NA
Type II+

Adhering to these water quality standards is crucial for successful cell culture experiments. Type I water is recommended for critical cell culture applications, while Type II water may be suitable for routine experiments.

NOTES:

If removal of finer particulates (< 0.05 micron) is required, consider upgrading to CLS-5400 series systems.
Applications requiring Endotoxin filtration should purchase a CLF-5000-02E Endotoxin removal final filter.

CLS-3300

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Download Spec Sheet

CLR 3300 High Purity Lab Water System with 0.05 Micron Ultrafiltration

25.25

⅜" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 10 CFU / ml

< 1ppb

< 15 ppb

Countertop, Wall Mount

Cell Culture, Electrophoresis, Immunochemistry, Life Sciences, Microbiology

Biologic Chemistry

CLS-5300-R-1

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CLïR 5300 Ultrapure Lab Water System with Ultrafiltration (Remote 120v)

14.4

21.6

14.7

⅜" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

< 15 ppb

Wall Mount, Remote Dispense

Cell Culture, Electrophoresis, Immunochemistry, Life Sciences, Microbiology

Biologic Chemistry

CLS-5300-R-2

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CLïR 5300 Ultrapure Lab Water System with Ultrafiltration (Remote 220v)

14.4

21.6

14.7

⅜" O.D. Tubing

220V/50Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

< 15 ppb

Wall Mount, Remote Dispense

Cell Culture, Electrophoresis, Immunochemistry, Life Sciences, Microbiology

Biologic Chemistry

CLS-5300-S-1

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Download Spec Sheet

CLïR 5300 Ultrapure Lab Water System with Ultrafiltration (Standard 120v)

14.4

21.6

14.7

⅜" O.D. Tubing

120V/60Hz

> 18 MΩ/cm

< 1 CFU/ml

< 1ppb

< 15 ppb

Countertop

Cell Culture, Electrophoresis, Immunochemistry, Life Sciences, Microbiology

Biologic Chemistry

System

Label

System Depth (in)

System Height (in)

System Width (in)

Dry Weight (lbs)

Inlet Connection

System Voltage

TOC Monitoring

0.05μ Ultrafiltration

Ultraviolet Oxidation

Effluent Purity

Effluent Microorganisms

Effluent Sodium

Effluent Chlorides

Effluent TOC

System Maximum
Output (lpm)

Volumetric Presets

Mounting

Laboratory Applications

The difference is CLïR.

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Applications

Biologic Chemistry

Cell Culture

What Is The Impact Of Water On Cell Culture?

Bacteria

Endotoxins

Organic Compounds

Ionic Contaminants

Water Measurement Requirements for Cell Culture

Application

Resistivity (MΩ.cm)*

TOC (ppb)

Filter (μm)

Bacteria (CFU/ml)

Endotoxins (EU/ml)

Nucleases

Water Grade

Application

Resistivity (MΩ.cm)*

TOC (ppb)

Filter (μm)

Bacteria (CFU/ml)

Endotoxins (EU/ml)

Nucleases

Water Grade

Application

Resistivity (MΩ.cm)*

TOC (ppb)

Filter (μm)

Bacteria (CFU/ml)

Endotoxins (EU/ml)

Nucleases

Water Grade

Application

Resistivity (MΩ.cm)*

TOC (ppb)

Filter (μm)

Bacteria (CFU/ml)

Endotoxins (EU/ml)

Nucleases

Water Grade

Application

Resistivity (MΩ.cm)*

TOC (ppb)

Filter (μm)

Bacteria (CFU/ml)

Endotoxins (EU/ml)

System

Label

System Depth (in)

System Height (in)

System Width (in)

Dry Weight (lbs)

Inlet Connection

System Voltage

TOC Monitoring

0.05μ Ultrafiltration

Ultraviolet Oxydation

Effluent Purity

Effluent Microorganisms

Effluent Sodium

Effluent Chloride

Effluent TOC

System MaximumOutput (lpm)

Volumetric Presets

Mounting

Laboratory Applications

CAtegory

System

Label

System Depth (in)

System Height (in)

System Width (in)

Dry Weight (lbs)

Inlet Connection

System Voltage

TOC Monitoring

0.05μ Ultrafiltration

Ultraviolet Oxydation

Effluent Purity

System Maximum
Output (lpm)

System Maximum
Output (lpm)

System Maximum
Output (lpm)

System Maximum
Output (lpm)